Histology

• Preparation of Mowiol mounting medium [Download pdf]
• In situ hybridization to mRNA in plant tissue sections
• Isolation of trichomes [Download pdf]
• Indirect immunofluorescence detection of F-actin and microtubules [Download pdf]

Biolistic transformation of onion epidermal cells

General lab protocols

Rapid plasmid preparation [Download pdf]

Preparation of competent cells

RNA quantification [Download pdf]

Plant RNA isolation by CsCl centrifugation [Download pdf]