My research was done under PhD candidate Clayton Cox and Dr. Max Teplitski in the Microbial Ecology lab at the Genetics and Cancer research center. Basically, we try to teach γ-proteobacteria how to behave.
This is done is by screening chemical libraries in an attempt to find inhibitors for a regulatory system that these pathogens used to initiate a cascade of physiological changes that result in the expression of behavioral traits (biofilm formation, stress response, SP-1 transcription) critical for enabling a bacteria to exhibit the deleterious behaviors of a pathogen.. The benefit in targetting this regulatory system (called GacS/GacA) is that while the regulator enables the bacteria to exhibit certain advantageous behaviors believed to be linked to colonization of a host and resistance to environmental insults such as host immune responses, the regulator is not necessary for a cell life cycle. Mutants lacking this regulator exhibit complete life cycles without expressing the traits controlled by this sytem. This type of treatment will theoretically enable us to reduce pathogen conatimation in common food vectors without placing the bacteria under environmental pressure to evolve around our treatment. These screens may also help us locate the cognate signal GacS/GacA responds to, a signal that is currently unknown.