Monoclonal Antibodies

mAb
Antigen
Application
Application Notes
28F2 Nop1p Marker for nucleolus in budding yeast
28F2 recognizes Nop1p in yeast but not fibrillarin in other species.
28F2 recognizes Nop1p in yeast more strongly than does mAb 38F3.
28F2 works for blots, immunofluorescence, and immunoprecipitation. 
38F3
Fibrillarin
Marker for nucleolus in many species 38F3 recognizes fibrillarin in many species, including human, rat, mouse, plant, fly, worm, fission yeast, and budding yeast (i.e., Nop1p).
38F3 works for blots, immunoprecipitation, and immunofluorescence.
39C7
Nups
Marker for nuclear pore complex in mammalian cells and yeast cells
39C7 recognizes multiple nucleoporin proteins present in nuclear pore complexes in budding yeast and mammalian cells.
39C7 works for blots and immunofluorescence.
40B7
Pma1p
Marker for plasma and vacuolar membranes in budding yeast
40B7 works for blots and immunofluorescence.

Antigen Information

Monoclonal Antibody Preparation

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Background image:  color inverted micrograph of budding yeast cells stained
with mAb 39C7 to detect nuclear pore complexes (violet) and DAPI to
detect chromatin in the nucleus and mitochondria (green).